opuAA [2017-11-24 14:02:38]

glycine betaine and arsenobetaine [SW|ABC transporter] (ATP-binding protein)

locus: BSU02980

pI: 5.11

mw: 46.28 kDa

protein length: 418 aa

gene length: 1254 bp

function: compatible solute transport

product: glycine betaine [SW|ABC transporter ](ATP-binding protein)

essential: no

ec: 3.6.3.32

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 321,013 → 322,269

The protein

Catalyzed reaction/ biological activity

• uptake of glycine betaine and arsenobetaine [pubmed|29159878]
• uptake of dimethylglycine [Pubmed|24561588]

Protein family

• [SW|ABC transporter] domain (according to Swiss-Prot)

[SW|Domains]

• 2 [SW|CBS domain]s (284-340, 344-403)

[SW|Localization]

• associated to the membrane (via [protein|5F989C57010ACFC03E37AF5A894153F432520921|OpuAB]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]-[gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]-[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23175650], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

  regulation

  • induced by osmotic stress [Pubmed|23175650]

Biological materials

Mutant

• BKE02980 (Δ[gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCCTCCATATTA, downstream forward: _UP4_CCTTCTGCACAGGAGGTGAA
• BKK02980 (Δ[gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTTCCCTCCATATTA, downstream forward: _UP4_CCTTCTGCACAGGAGGTGAA

Expression vector

• pGP2924 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 27935846

Original publications

• 7622480,10092453,16645306,14623183,18321243,16225868,21296969,22383849,23175650,23646920,24561588,29159878